USA Surgery Today

Children and adults rely on the release of energy from ingested nutrients or established body stores to fuel the work of the human body. In this way, the energy derived from the degradation of large substrate molecules is used to synthesize high-energy phosphate bonds in the form of adenosine triphosphate (ATP). The bulk of energy metabolism in the human is performed in the aerobic setting, although anaerobic pathways exist and indeed are accentuated in the setting of stress or disease. The hydrolytic cleavage of a phosphate group from ATP, thereby producing adenosine diphosphate, provides much of the energy needed for cellular reactions.

The major sources of energy to fuel the processes of work are carbohydrate, lipid, and protein. These substrates are degraded by the body into the currency of energy metabolism: glucose, fatty acids, and amino acids. For most tissues, fatty acids are the major source for oxidative energy production. However, glucose is of great importance because it is the preferred energy source for cells in the central nervous system, renal medulla, and erythrocytes. These tissues cannot take up fatty acids from the systemic circulation, and therefore rely on glucose or ketone bodies during starvation. Pathways exist to synthesize glucose from the breakdown of amino acid and lipid substrates, a process that is accelerated in critical illness. In general, the breakdown and synthesis of glucose, lipid, and protein are tightly controlled by neural, hormonal, and allosteric regulation.

In the fasting state, the body mobilizes host substrate to provide energy in the form of glucose for the continuation of cellular work. Glycogen, the storage form of glucose found in liver and skeletal muscle, is the first substrate to be broken down for glucose production. Glycogen stores are limited in both the adult and child, and are effectively depleted within 24 hours. At this point, host protein stores, and to a lesser extent the glycerol backbone of triglycerides, must be mobilized for the continued supply of glucose. The glucose that is produced may be used in a variety of ways: (1) transported to the brain, kidney, and erythrocyte for cellular energy; (2: Capoten ) catabolized via glycolysis and the citric acid cycle, and ultimately used in oxidative phosphorylation for ATP production; or (3: Capoten ) in the anaerobic setting, degraded via glycolysis for limited energy and ATP production. Glycolysis in the anaerobic setting is responsible for the production of lactic acid from pyruvate. In the Cori cycle, lactate produced from peripheral tissues is transported to the liver, where it can be converted back to pyruvate and used to produce glucose. In this way, during anaerobic metabolism, the liver can continue to supply extrahepatic tissues with glucose while peripheral tissue can use glycolysis for a limited energy supply. The yield from the catabolism of one molecule of glucose from oxidative phosphorylation is 38 molecules of ATP versus only 2 molecules of ATP in glycolysis. Clearly, aerobic oxidation of glucose is the preferred pathway for energy production in the human.

When glycogen has been depleted, lipid is the next substrate to be mobilized for energy production. Triglycerides stored in the cell are broken down and transported in the form of fatty acids to the liver, muscle, and heart. Here, individual fatty acids are degraded in the process of β-oxidation, which produces one acetyl-coenzyme A (acetyl-CoA: Capoten ) molecule for every two carbon groups in the fatty acid. Acetyl-CoA can then be oxidized further through the citric acid cycle and ultimately used to produce ATP via oxidative phosphorylation. Acteyl-CoA in mammalian tissue cannot undergo conversion to glucose. The glycerol backbone of the triglyceride is converted to an intermediate in glycolysis and can also be used for energy production or gluconeogenesis.

In settings of extreme fasting or uncontrolled diabetes mellitus, the metabolic pathways are regulated to prioritize the production of glucose for fuel to extrahepatic tissues. The accelerated production of glucose depletes the hepatocyte of needed intermediates in the citric acid cycle. When this occurs, the acetyl-CoA generated from the breakdown of fatty acids cannot enter the citric acid cycle and instead forms the ketone bodies, acetoacetate and hydroxybutyrate, through a nonenzymatic condensation reaction. These ketone bodies are released by the liver to extrahepatic tissues, including skeletal muscle and the brain, where they can be used for energy production. In these peripheral tissues where the citric acid cycle remains active, ketone bodies enter the citric acid cycle for ATP production. The system of ketone body formation allows continued oxidation of fatty acids in the liver for the provision of energy to peripheral tissue when glucose is not available. During surgical illness, insulin levels are elevated and hence ketone body formation is relatively inhibited. Thus, ketone bodies do not significantly supplant the need for glucose in surgical patients.

Under normal conditions, protein stores are not a primary substrate for the generation of energy in the human body.(- Capoten-) Rather, protein serves as the body’s major component for synthesis and structure. Proteins, and their constituent amino acids, however, are never static and exhibit a “turnover” due to constant degradation and synthesis. In settings of critical illness and injury, protein breakdown is accelerated significantly. When amino acids must be substituted as a substrate for energy production, the body gradually loses its structural protein stores. Net protein loss in critical illness must be avoided in order to maintain the host’s capacity to heal wounds and avoid infection. In children, protein loss halts growth and normal development.

When amino acid catabolism occurs, the initial step involves removal of the toxic amino group (NH₃) from the amino acid. Through transamination, the NH₃ is transferred to -ketoglutarate, thereby producing glutamate. The addition of an additional amino group converts glutamate to glutamine, which is then transported to the liver. Here, the amino groups are removed and the ammonia detoxified to urea via the urea cycle. Alternatively, in muscle tissue the amino group can be transferred to pyruvate, forming the amino acid alanine. When alanine is detoxified in the liver, pyruvate is thus reformed and can be converted into glucose via gluconeogenesis. The transport of alanine between peripheral tissue and the liver for ammonia detoxification and glucose generation is termed the glucose-alanine cycle. Hence, the transport amino acid systems involving glutamine and alanine provide carbon backbones for gluconeogenesis in the liver while they facilitate the detoxification of ammonia by the urea cycle. ( Capoten)

In the postprandial state, the glucose, amino acids, and fatty acids absorbed from the small intestine are distributed by the liver for appropriate energy metabolism. The majority of fatty acids are packaged by the enterocyte into chylomicrons and excreted into the lymphatic system. These lipid molecules will be stored as triglycerides or phospholipids in adipose tissue. The remaining medium-chain fatty acids are transported to the hepatocyte with glucose and amino acids via the portal circulation. The liver oxidizes fatty acids to form acetyl-CoA, which can enter the citric acid cycle and proceed to oxidative phosphorylation. Tissues that cannot take up fatty acids from the systemic circulation—the brain, renal medulla, and red blood cells—rely primarily on glucose for energy needs.

Glucose can be shunted to several different pathways by the liver following a meal. If blood sugar is low, some glucose will be released into the systemic circulation to maintain glucose homeostasis. Alternatively, glucose can be phosphorylated to glucose-6-phosphate, an intermediate of multiple pathways. Glucose-6-phosphate can be (1: Capoten ) used for glycogen production to store excess glucose, (2) converted to acetyl-CoA by glycolysis and subsequent energy production via the citric acid cycle and oxidative phosphorylation, (3: Capoten) converted to acetyl-CoA and shunted toward lipid biosynthesis, or (4) oxidized in the pentose phosphate pathway for ultimate conversion to steroid precursors and nucleotides.

Amino acids in the hepatocyte can be used for the synthesis of both hepatic and extrahepatic proteins, including structural proteins and synthetic enzymes. Extra supplies of amino acid in the hepatocyte are catabolized to citric acid precursors and degraded for oxidative energy production.

The process of substrate metabolism is similar in adults and children, although noteworthy differences exist. Neonates in particular have a high demand for glucose, which may be secondary to their increased brain-to-body mass ratio. In addition, glycogen stores are relatively depleted in the neonate, particularly in the preterm infant (5: Capoten ). Clinically, short periods of fasting can predispose the newborn infant to hypoglycemia. Thus, in these patients, the body must turn to the breakdown of lipid and protein stores earlier in the process of substrate metabolism to provide an effective energy source. However, as the majority of polyunsaturated fatty acids accumulate toward the end of pregnancy, the premature child has a lower proportion of lipid stores. Neonates also have decreased lipid stores as compared with the adult, thereby rendering lipid less useful as a potential fuel source (6). Taken together, the neonate’s increased energy needs and limited substrate stores mandate early nutritional support in settings of critical illness

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Capoten GN:Captopril (KAP toe pril) BN:Capoten